Serial Crystallography with Multi-stage Merging oi 1000's of Images (Q6693175)

In the associatedpaper we discussthe issues involved in improving the sensitivity of both approaches to clustering, using, as an example, 999 5degreewedges from lysozyme in four iso-forms: NAG: native with N-acetylglucosamine (NAG) soaked in, benzamadine: native with benzamadine soaked in, benzamadine plus NAG: native with both NAG and benzamadine soaked in. Native: no ligands Although the cell parameters are changed sufficiently to allow recognition of the NAG soak,it is difficult to filter the benzamadine soak simply on the basis of cell parameter changes, suggesting the desirability of switching from cell-based clustering to reflection-based clustering as early in the process as possible. The data is this dataset consists of a bzippedHKL structure factor tarballand a bzippedcluster data files from the four forms tarball.