moFluMemB - Dataset : scRNA-seq from Lymph node, Spleen and Lung - 10x_191105_m_moFluMemB (Q6709121)

Title Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanismAuthorsClaude Gregoire,1 Lionel Spinelli,1 Sergio Villazala-Merino,1 Laurine Gil,1 Mara Pa Holgado,1 Myriam Moussa,1 Chuang Dong,1 Ana Zarubica,2 Mathieu Fallet,1 Jean-Marc Navarro,1 Bernard Malissen,1,2 Pierre Milpied,1,* and Mauro Gaya1,*Affiliations1 Centre d'Immunologie de Marseille-Luminy (CIML), Aix Marseille Universit, INSERM, CNRS, Marseille, France2 Centre d'Immunophnomique (CIPHE), Aix Marseille Universit, INSERM, CNRS, Marseille, France* Correspondence: milpied@ciml.univ-mrs.fr (P.M.), gaya@ciml.univ-mrs.fr (M.G.)SummaryLung-resident memory B cells (MBCs) provide localized protection against reinfection in the respiratory airways. Currently, the biology of these cells remains largely unexplored. Here, we combined influenza and SARS-CoV-2 infection with fluorescent-reporter mice to identify MBCs regardless of antigen specificity. We found that two main transcriptionally distinct subsets of MBCs colonized the lung peribronchial niche after infection. These subsets arose from different progenitors and were both class-switched, somatically mutated and intrinsically biased in their differentiation fate towards plasma cells. Combined analysis of antigen-specificity and B cell receptor repertoire segregated these subsets into bona fide virus-specific MBCs and bystander MBCs with no apparent specificity for eliciting viruses and generated through an alternative permissive mechanism. Thus, diverse transcriptional programs in MBCs are not linked to specific effector fates but rather to divergent strategies of the immune system to simultaneously provide rapid protection from reinfection while diversifying the initial B cell repertoire. Data: 10x_191105_m_moFluMemB_processedData.tar.gz : pre-processed data of 10x 5 scRNA-Seq on memory B cells sorted from single-cell suspensions of spleen, lymph nodes and lungs with mechanical dissociation of lung tissue at 4C. See the three other Zenodo deposit for the rest of the data: 10.5281/zenodo.5566674 10.5281/zenodo.5564624 10.5281/zenodo.10559312