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PSFcheck ring pattern at various SNR - MaRDI portal

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PSFcheck ring pattern at various SNR

From MaRDI portal



DOI10.5281/zenodo.6955019Zenodo6955019MaRDI QIDQ6690329

Dataset published at Zenodo repository.

Author name not available (Why is that?)

Publication date: 20 July 2022

Copyright license: No records found.



Description

The imaged PSFcheck pattern consists of a nanometric set of ring-like laser-written structures with a separation of 10 m between each. The mean FWHM of these patterns was calculated to be 208 nm. A NanoImager-S microscope (ONI, Oxford Nanoimaging), equipped with a 100X 1.4 NA, oil-immersion objective (Olympus) was used to image the PSFcheck patterns in widefield fluorescence mode. Sample excitation was provided by a 561 nm laser and emission was collected with a 575-616.5 emission filter. A sCMOS sensor (Hamamatsu, Orca-0Flash4.0 V3) was used for image aquisition at 30 fps and 117 nm pixel size.






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