Conformational changes in the connector protein complex of the bacteriophage \(\varphi 29\) DNA packaging motor
type: Item
instance of: scholarly article
title: Conformational changes in the connector protein complex of the bacteriophage \(\varphi 29\) DNA packaging motor
zbMATH Open document ID: 1154.92313
DOI: 10.1080/17486700802168486
author: Arron C. Tolley
author: Nicola J. Stonehouse
published in: Computational \& Mathematical Methods in Medicine
publication date: 2009-02-18T00:00:00Z
full work available at URL: https://eudml.org/doc/232833
review text: Summary: DNA packaging in the bacteriophage \(\varphi 29\) involves a molecular motor. It is proposed that dsDNA is packaged through a channel in a connector located at the 5-fold vertex of a preformed prolate icosahedral capsid. The packaging motor also consists of virally-encoded RNA molecules (pRNA) coupled to ATPases. Data obtained from studies using surface plasmon resonance, fluorescence quenching and circular dichroism are presented to demonstrate the importance of the N-termini of the connector protein subunits in pRNA interactions and in conformational change. Based on our findings, we propose a model of DNA packaging based on connector conformational change.
Mathematics Subject Classification ID: 92C40
zbMATH DE Number: 5508961
zbMATH Keywords: RNA-protein interaction
zbMATH Keywords: bacteriophage \(\varphi 29\)
zbMATH Keywords: molecular motor
zbMATH Keywords: virus assembly
zbMATH Keywords: conformational change
MaRDI profile type: Publication
OpenAlex ID: W2124618236
description: scientific article; zbMATH DE number 5508961