Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
type: Item
instance of: scholarly article
title: Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
zbMATH Open document ID: 1423.92174
DOI: 10.1155/2014/820214
author: Pedro L. Rodrigues
author: Nuno F. Rodrigues
author: Duarte Duque
author: Sara Granja
author: Jorge Correia-Pinto
author: João L. Vilaça
published in: Computational \& Mathematical Methods in Medicine
publication date: 2015-03-16T00:00:00Z
review text: Summary: \(Background.\) Regulating mechanisms of branching morphogenesis of fetal lung rat explants have been an essential tool for molecular research. This work presents a new methodology to accurately quantify the epithelial, outer contour, and peripheral airway buds of lung explants during cellular development from microscopic images. \(Methods.\) The outer contour was defined using an adaptive and multiscale threshold algorithm whose level was automatically calculated based on an entropy maximization criterion. The inner lung epithelium was defined by a clustering procedure that groups small image regions according to the minimum description length principle and local statistical properties. Finally, the number of peripheral buds was counted as the skeleton branched ends from a skeletonized image of the lung inner epithelia. \(Results.\) The time for lung branching morphometric analysis was reduced in 98\% in contrast to the manual method. Best results were obtained in the first two days of cellular development, with lesser standard deviations. Nonsignificant differences were found between the automatic and manual results in all culture days. \(Conclusions.\) The proposed method introduces a series of advantages related to its intuitive use and accuracy, making the technique suitable to images with different lighting characteristics and allowing a reliable comparison between different researchers.
Mathematics Subject Classification ID: 92C55
zbMATH DE Number: 6415233
zbMATH Keywords: lung branching morphogenesis
zbMATH Keywords: fetal rat explants
zbMATH Keywords: automated image analysis
MaRDI profile type: Publication
full work available at URL: https://doi.org/10.1155/2014/820214
OpenAlex ID: W1986906016
Wikidata QID: Q34177193
DBLP publication ID: journals/cmmm/RodriguesRDGCV14
description: scientific article